FIGURE 2.

Steady state levels of Om14p, Om45p, and Por1p in deletion strains. 10 μg of mechanically isolated mt proteins from the deletion strains Δom14 OM45-cMyc, Δom45 OM14-HA, Δpor1 OM14-HA (Δpor1-1), Δpor1 OM45-cMyc (Δpor1–2), Δom14 Δom45, and control strain OM14-HA OM45-cMyc were separated on a 15% SDS-polyacrylamide gel and analyzed by Western blot. Specific primary and Cy5-conjugated secondary antibodies were used. Fluorescence images from three independent experiments were quantified using ImageQuant Version 5.2 software. Signals were correlated with the intensity of the Cox2p signal, which served as a control, and values were normalized to control strain (100%).