FIGURE 3.

Molecular organization of Om14p, Om45p, and Por1p in deletion strains. 200 μg of highly purified mt from the strains Δom45 OM14-HA, Δpor1 OM14-HA (Δpor1-1), Δom14 OM45-cMyc, Δpor1 OM45-cMyc (Δpor1-2), Δom14 Δom45, and OM14-HA OM45-cMyc (control) were lysed with 4% digitonin. High molecular weight complexes were separated by BN-PAGE in a 3–13% gradient gel. Subunit composition of complexes was assessed by a second dimension under denaturing conditions (12% SDS-PAGE). Proteins were transferred onto a PVDF membrane. For detection of Om14p (A), Om45p (B), and Por1p (C), antibodies against HA-, cMyc-tag, and Por1p, respectively, were used. Determination of molecular weight was done with the high molecular weight calibration kit.