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. 2012 Mar 29;287(21):17447–17458. doi: 10.1074/jbc.M111.328328

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© 2012 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 4. — Por1p and Om14p interact irrespective of Om45p. Co-IPs were performed with mt lysates of the deletion strains Δom45 OM14-HA (A), Δom14 OM45-cMyc (B), and Δpor1 OM14-HA OM45-cMyc (C) in combination with the indicated immobilized antibodies against Por1p, HA-, or cMyc-tag, respectively. Proteins of the final eluates and an aliquot of the corresponding supernatant of lysed mt (S) were separated by 12% SDS-PAGE and transferred onto a PVDF membrane prior to immunodetection with specific antibodies against Por1p, Om14p-HA, and Om45p-cMyc. As controls, antibodies against the OM proteins Tom22p and Tom40p and the IM protein Cox2p were used.