TABLE 2.

Changes of the mt proteome of the strains Δom14, Δom45, and Δom14 Δom45 in comparison to wild type

Protein spots with significant changes (threshold, 3-fold; −, decreased; +, increased) of their fluorescence intensity in comparison to wild type in the two-dimensional DIGE gels (see Fig. 7) were analyzed by MALDI-TOF/TOF MS (see under “Experimental Procedures” and supplemental Table S2). C, cytoplasm; M, mitochondria; MA, mt matrix; SGD, Saccharomyces Genome Database.

Spot no.	Protein name	Abundance change	Function/process	Localization	Co-factor/substrate	Carrier for molecule	Ref.
Δom14
1	Tdh1p	−4.3	Glycolysis/glyconeogenesis	C	NAD+/NADH	Ndt1/2p	6, 45
2	Ynl208w-like	−3.2	Unknown	M			SGD
3	Lsc1p	−3.1	Citric acid cycle	MA	ADP	Aac1/2/3p	6, 36, 46
					Pi	Mir1p
Δom45
4	Om45p-1	−24.6	Unknown	OM			SGD
5	Om45p-4	−10.1	Unknown	OM			SGD
6	Om45p-2	−6.6	Unknown	OM			SGD
8	Om45p-3	−6.4	Unknown	OM			SGD
15	Ccp1p-2	−5.8	Oxidative stress response	IMS	Heme	Aac1/2/3p	48, 54
7	Tef2p	−4.8	Translation elongation factor	C	GDP/GTP	Ggc1p	6, 47
9	Ccp1p-1	−4.1	Oxidative stress response	IMS	Heme	Aac1/2/3p	48, 54
Δom14Δom45
1	Tdh1p	−18.2	Gluconeogenesis	C	NAD+/NADH	Ndt1/2p	6, 45
4	Om45p-1	−16.0	Unknown	OM			SGD
5	Om45p-4	−10.0	Unknown	OM			SGD
8	Om45p-2	−6.8	Unknown	OM			SGD
7	Tef2p	−5.9	Translation elongation factor	C	GDP/GTP	Ggc1p	6, 47
10	Pst2p	−4.7	Stress response	M	FMNa	Unknown	49, 50, 56, 57
6	Om45p-3	−4.5	Unknown	OM			SGD
11	Qcr2p	−3.7	Respiratory chain	IM	Zn2+a	Unknown	51, 58, 83
3	Lsc1p	−3.5	Citric acid cycle	MA	ADP	Aac1/2/3p	6, 46
12	Lsp1p	−3.4	Heat stress response	C	Pi	Mir1p	52
13	Om14p/Pam17p	−3.0	Unknown	OM/IM			SGD

^a This is an assumption.