FIGURE 5.
Amiloride sensitivity of truncated MCaUF peptide cell entry. A, representative FACS analyses of the effect of 5 mm amiloride on MCaUF1–33-C-Cy5 (upper left), MCaUF1–15-C-Cy5 (upper right), MCaUF20–33-C-Cy5 (lower left), and MCaUF18–33-C-Cy5 (lower right) entries. Numbers in red represent average decrease or increase in peptide entry upon amiloride treatment. Cells were treated for 2 h with 3 μm peptide concentration with or without 5 mm amiloride. a.u., arbitrary unit. B, average effect of amiloride on mean cell entry of the truncated peptides. Positive values reflect increase in cell entries, whereas negative values indicate reduction in cell penetration.