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ITC experiments. A, maltose titrated into MalE; KD = 2.18 ± 0.091 μm, ΔH = 4.467 ± 0.037 kJ, ΔS = 41.8 J/K. B, maltose titrated into MalE and MalF-P2 (1:1.2 molar ratio); KD = 0.121 ± 0.043 μm, ΔH = 37.59 ± 0.849 kJ, ΔS = 166 J/K. All experiments were carried out at 5 °C. In the presence of MalF-P2 the apparent stoichiometry is reduced to ∼25%, indicating that one quarter of all MalE/MalF-P2 complexes are able to coordinate a maltose molecule.
