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Radioactive maltose precipitation experiments. All experiments were performed with equimolar amounts of MalE and maltose in the presence or absence of MalF-P2 at a 1:1 or 1:2 molar ratio. Radioactive counts from filter assays (20) of [14C]maltose were measured by a liquid scintillation counter. Experiments were performed in duplicate for each sample. No significant difference of free maltose in the supernatant is observed after precipitation of the protein.
