TABLE 2.

Ratio of solvent-PRE relaxation rates for substrate-bound MalE in presence and absence of MalF-P2

Relaxation rates of residues lining the binding pocket and/or directly associated with substrate binding were extracted and compared relatively with each other. A >5-fold increase in relaxation rates of residues located in the vicinity of the maltose-binding pocket is observed after addition of MalF-P2.

Residue	ϵMalE/MalF-P2/ϵMalE	Residue	ϵMalE/MalF-P2/ϵMalE
Lys15	5.78 ± 0.28	Val110	1.02 ± 0.43
Glu44	4.02 ± 0.20	Glu111	2.96 ± 0.38
Trp62	5.33 ± 0.30	Ala112	38.9 ± 0.40
Ala63	10.3 ± 0.28	Ser114	4.22 ± 0.31
His64	3.03 ± 0.29	Glu153	13.0 ± 0.34
Arg66	2.95 ± 0.54