FIGURE 4.

Thermal denaturation of TrwD followed by CD. Temperature dependence of the molar ellipticity at 222 nm of a sample containing 5 μm TrwD in 50 mm Hepes buffer (pH 8.5) without added magnesium (dark gray) and with 2 mm magnesium acetate (black) or 1 mm MgADP (gray), heated from 20 to 80 °C in a 0.1-cm path length cuvette at a rate of 60 °C/h, deg, degree.