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Strictly conserved phenylalanine residues within the FF4/FF5 domains are required for the targeting to nuclear speckles. Dual labeling of HEK293T cells transfected with TCERG1[1–662]-FF4/FF5 or the indicated phenylalanine-to-alanine mutant constructs (ECFP, green) with the anti-SC35 antibody (SC35, red) was performed. Individual and merged images of the cell are shown. Scale bars = 3 μm.
