FIGURE 6.
Deletion of the FF4/FF5 domains of TCERG1 affects Bcl-x alternative splicing. A, schematic representation of the structure of the Bcl-x minigene is drawn with exons (boxes) and introns (lines). The Bcl-x pre-mRNA is alternatively spliced (dotted lines) to produce two major isoforms, Bcl-xL and Bcl-xS. B, splicing assay of the Bcl-x minigene in HEK293T cells transfected with the indicated constructs. Cells were harvested ∼44 h after transfection and processed for RT-PCR. The data are presented as the ratio of Bcl-xL to Bcl-xS from three independent experiments (mean ± S.D.). *, p < 0.05.