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Kinetics parameters of Ahp1 and mutants
| Ahp1 |
t-BOOH |
Trx2 |
||||||
|---|---|---|---|---|---|---|---|---|
| Km | kcat | nH | kcat/Km | Km | kcat | nH | kcat/Km | |
| μm | s−1 | ×105m−1s−1 | μm | s−1 | ×106m−1s−1 | |||
| WT | 76.9 ± 5.5 | 8.4 ± 0.3 | 1.9 ± 0.1 | 1.1 | 1.3 ± 0.3 | 8.3 ± 0.6 | 1.1 ± 0.1 | 6.4 |
| K32R | 49.4 ± 3.4 | 5.7 ± 0.1 | 1.5 ± 0.1 | 1.2 | 1.6 ± 0.2 | 6.5 ± 0.3 | 1.0 ± 0.1 | 4.1 |
| K32A | 60.2 ± 9.4 | 5.0 ± 0.5 | 1.6 ± 0.2 | 0.8 | 2.4 ± 0.2 | 5.9 ± 0.2 | 0.9 ± 0.0 | 2.5 |
| K32E | 47.4 ± 3.6 | 3.9 ± 0.2 | 1.5 ± 0.1 | 0.8 | 2.8 ± 0.4 | 5.2 ± 0.3 | 1.0 ± 0.1 | 1.9 |
