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. 2012 Mar 29;287(21):17100–17108. doi: 10.1074/jbc.M111.316943

FIGURE 1.

FIGURE 1.

Effect of GITR ligation by GITRL on MAPK phosphorylation in nTregs. nTregs were isolated from the lungs of C57BL/6 mice and incubated with different concentrations of GITRL for varying lengths of time. Data illustrated are derived from densitometric quantification of autoradiographs using ImageJ. Levels of relative phosphorylation are derived from base-line values (nonstimulated, time 0) of the ratio of p-ERK to total ERK. A–C, dose and time course of GITR-mediated phosphorylation of JNK but not ERK or p38. D, GITR-mediated phosphorylation of JNK in CD4+CD25− T cells. PI, phorbol/ionomycin. Med, medium. Results are from three independent experiments carried out in duplicate. **, p < 0.01; *, p < 0.05.