FIGURE 6.

EPR spectra of the purified E. coli complex I from wild type and various NuoI mutants at 40K. . Representative traces from different group of mutants are as follows: WT (1), C63S (2), P71A (3), and P110A (4). The samples were reduced with 10 mm sodium dithionite in the presence of 5 μm of benzyl viologen and methyl viologen. EPR spectra were recorded under the following conditions: microwave frequency, 9.45 GHz; microwave power, 5 milliwatts; modulation amplitude, 8.034 G; modulation frequency, 100 kHz; time constant, 82 ms. The g_z and g_x values are indicated.