FIGURE 2.

Overexpression of S100A14 promoted cell invasiveness by regulating MMP2. A, expression of some metastasis-related genes was examined by RT-PCR in EC9706 transfectants. B, MMP2 mRNA expression levels were further confirmed by RT-qPCR. Western blot analysis showed the expression in cell lysates (C) and the secretion and activation of MMP2 in conditioned medium in EC9706 transfectants (D). E, EC9706 cells (P4, S12, and S13) were pretreated with 10 μm OA-Hy or solvent (DMSO) for 24 h and suspended in 150 μl of serum-free medium containing OA-Hy or solvent. Cells were then seeded in the upper chamber of the Matrigel plate for the invasion assay. Left panel, representative pictures of invading cells. Right panel, relative numbers of invading cells. Cells were counted in four randomly selected fields. Error bars represent the S.E. of triplicate experiments. *, p < 0.05 two-tailed Student's t test.