FIGURE 4.

ET-1 is rapidly internalized by caveolae in cultured endothelial cells. Images from confocal fluorescence microscopy of tetramethylrhodamine-ET1 localization (red) on intact BAEC immediately fixed after 4 °C binding (A) or after the cells were warmed to 37 °C for 5 min (B) prior to fixation with paraformadehyde. The cells were then permeabilized before incubation with cav1 antibodies and fluorophore-conjugated reporter antibodies (cav1, green). The bar represents 50 μm.