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. Author manuscript; available in PMC: 2012 Jun 4.
Published in final edited form as: Biochemistry. 2008 Jul 18;47(32):8456–8464. doi: 10.1021/bi8007643

Figure 3.

Figure 3

S1334T mutation increases the Km (ATP) for ATPdependent LTC4 transport. Membrane vesicles prepared in Figure 2 were used to do the ATP-dependent LTC4 transport, according to the methods described in Experimental Procedures. Briefly, the assays were carried out in a 30 μL solution containing 3 μg of membrane vesicles, 10 mM MgCl2, and varying concentrations of ATP at 37 °C for 1 min. Since the amounts of MRP1 proteins determined in Figure 2A were different, the amounts of membrane vesicles used in these experiments were adjusted to a similar amount by adding varying amounts of membrane vesicles prepared from parental BHK cells: 3 μg of wild-type MRP1; 2.308 μg of S1334T plus 0.692 μg of BHK. The data are the means ± SD of triplicate determinations.