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Pattern of PBPs in L. monocytogenes strain overexpressing lmo1438. Membrane proteins (200 μg of total protein) of L. monocytogenes pAKB (lane 1) and L. monocytogenes pAKB-lmo1438 (lane 2) were incubated with [3H]benzylpenicillin at a saturating concentration of 5 μg/ml and the radiolabeled PBPs were separated by SDS-PAGE and detected by fluorography. The PBP corresponding to each band is indicated on the right.
