. 2012 Apr 18;12:57. doi: 10.1186/1471-2180-12-57

Table 2.

PCR primers used in this study


Primer	Sequence (5'→ 3')

HlyA^a	GCGGGTACCAGGTAGAGCGGACATCCATTG
HlyB^{b, c, d}	GTTTTAGGATCCCCCGGGGGGTTTCACTCTCCTTCTAC
HlyC^{b, c}	CCCGGGGGATCCTAAAACCGCTTAACACACACG
HlyD^e	GCGTCTAGATTCTTCCCCGACAGAATCTGC
NisR F	CCCACTAAACAATCGGAGG
NisK R^c	GCGGGATCCCAGAAATTAAACCAAACAAAATTTTC
Oepbp3 F	CGTGAAACTAAATTTTAGAAAAAAGAAAAAAG
Oepbp3 R^f	GCGGCATGCGATTAATTTTCGGTTTGTTCTGATTG

^aNucleotide substitutions to create KpnI site are underlined

^bNucleotide substitutions to create SmaI site are underlined

^cNucleotide substitutions to create BamHI site are in boldface

^dOverhang complementary to SOE primer is in italics

^eNucleotide substitutions to create XbaI site are underlined

^fNucleotide substitutions to create SphI site are underlined