Figure 3.
Acetic acid (A) and xylitol (B) produced as a by-product during bioethanol production by different strains of Fusarium oxysporum cultivated on a straw/bran lignocellulosic substrate (10:1 ratio of straw to bran). Fungal isolates were grown in solid-state culture on 1 g straw/bran supplemented with 5 ml-1 minimal medium, pH 7 (Mishra et al. 1984) (initial moisture content was 91% vw-1). Cultures were incubated at 35°C for 4 days of aerobic followed by 4 days of oxygen-limited conditions. Acetic acid and xylitol content of the culture supernatant were determined enzymatically using the Megazyme™ acetic acid and Xylitol assay kit respectively (Megazyme, Ireland) according to manufacturer's instruction. Bars indicate SEM (LSD0.05 acetic acid = 0.45478; LSD0.05 xylitol = 0.3004)