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. 2012 Mar 15;2:16. doi: 10.1186/2191-0855-2-16

Figure 4.

Figure 4

Specific activity of the major cellulases, xylanase and alcohol dehydrogenase produced by strains of Fusarium oxysporum during growth on wheat straw/bran. Specific activity of (A) endoglucanase (EC 3.2.1.4), (B) exoglucanase (EC 3.2.1.91), (C) β-glucosidase (EC 3.2.1.21), (D) endoxylanase (EC 3.2.1.8), (E) β-xylosidase (EC 3.2.1.37) and (F) alcohol dehydrogenase (ADH) (EC 1.1.1.1) as determined for F. oxysporum strains 11C, 12A, 13C, 27E, 4E and 7E and commercial enzyme preparations, i.e. Celluclast®, Novozyme 188, Commercial mix. (83% vv-1 Celluclast®, 17% vv-1 Novozyme 188) and ADH (Sigma Chemical, St. Louis, USA). Fungal isolates were grown in solid-state culture on minimal medium, pH 7 (Mishra et al. 1984) supplemented with 1 g milled straw/bran (initial moisture content was 91% vw-1). Cultures were incubated at 30°C. Activities of cellulases enzymes in the water extract of the fermented straw (after 4 days of aerobic growth) were determined as described earlier by Wood & Bhat (1986) and Thygesen et al. (Thygesen et al. 2003). ADH activity in the cellular extract of the fermented straw (after 4 days of aerobic and 4 days oxygen-limited growth) were determined as described earlier by Kayali et al. (Kayali et al. 2005) and Ke et al. (Ke et al. 1995). Total protein in the extracts were measured by Bradford assay (Bradford 1976) and specific activity of the enzymes expressed as nkat μg-1of crude protein. Bars indicate SEM (LSD 0.05 for parts A - F = 89.92, 6.85, 0.52, 962.96, 0.48 and 60.41, respectively)