Table 3. Engraftment levels of human cells in peripheral blood (PB), spleen, lymph nodes (LNs) and bone marrow (BM) of humanized NSG mice.
| Weeks afterCD34+ cellstransplantation | 12-Weeks | 22-Weeks | |||||||||||
| Cell Percentage | % Murine CD45+ | % Human CD45+ | % CD19+ofhumanCD45 | % CD3+ofhumanCD45 | % CD4+ofhumanCD45 | % CD8+ofhumanCD45 | % Murine CD45 | % Human CD45 | % CD19+ofhumanCD45 | % CD3+ofhumanCD45 | % CD4+ofhumanCD45 | % CD8+ofhumanCD45 | |
| PB | Group 1Group 7 | 71.40±6.70% | 28.26±8.70% | 87.60±3.47% | 4.52±2.08% | 1.43±0.98% | 1.69±0.68% | 58.74±7.70% | 41.26±5.70% | 51.60±4.21% | 35.60±5.87% | 23.43±0.98% | 12.69±2.78% |
| 21.74±3.79% | 78.26±6.89% | 79.32±3.18% | 13.45±1.90% | 4.13±1.78% | 6.91±2.18% | 8.03±2.70% | 91.97±4.10% | 22.52±3.08% | 67.29±4.88 | 48.81±6.78% | 19.39±4.13% | ||
| Spleen | Group 1Group 7 | 78.34±4.07% | 21.66±3.70% | 91.90±4.51% | 3.52±0.88% | 1.11±0.98% | 1.89±0.68% | 68.34±8.10% | 31.66±4.30% | 68.60±5.21% | 20.91±6.17% | 8.43±2.08% | 12.69±1.18% |
| 41.74±3.07% | 58.26±3.99% | 83.32±4.18% | 10.15±1.91% | 3.03±0.13% | 7.12±1.17% | 25.69±2.17% | 74.31±5.92% | 52.52±6.88% | 42.29±4.88% | 16.81±6.85% | 12.39±4.43% | ||
| LNs | Group 1Group 7 | − | − | − | − | − | − | 24.84±3.30% | 75.16±13.30% | 38.60±6.51% | 50.66±3.87% | 24.03±4.98% | 14.69±2.78% |
| 11.84±2.31% | 88.16±7.09% | 73.11±4.18% | 21.15±3.90% | 7.53±1.78% | 12.99±3.18% | 4.49±0.70% | 95.51±3.23% | 21.52±2.98% | 77.29±11.88 | 48.81±6.18% | 21.09±4.43% | ||
| BM | Group 1Group 7 | 74.67±6.07% | 25.33±7.70% | 85.11±6.47% | 3.33±1.18% | 0.83±0.99% | 1.89±0.78% | 61.14±11.0% | 38.86±4.45% | 54.10±3.23% | 40.60±4.87% | 22.43±3.98% | 16.69±2.78% |
| 32.84±4.10% | 67.16±8.99% | 74.13±4.41% | 11.23±2.90% | 4.13±1.33% | 5.91±11.88% | 16.59±3.10% | 83.41±2.65% | 40.32±10.08% | 55.29±5.18 | 38.81±6.78% | 14.39±4.13% | ||
Peripheral blood and organs were collected at 12 and 22 weeks after CD34+ cell transplantation in group 1 and group 7 mice. Percentage of human and murine CD45 cells was calculated on lymphoid gate. Percentage of human CD19+, CD3+, CD4+ and CD8+ cells were calculated out of human CD45+ cells.