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. 2012 Jun 4;7(6):e38259. doi: 10.1371/journal.pone.0038259

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Liu et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Panel A shows the time courses of the increase in IAANS fluorescence as Ca²⁺ was removed by EGTA from control , and the DCM mutants, TnI K36Q , TnT R131W , TnT R141W , TnT R205L and TnT ΔK210 . Panel B shows the time courses of the increase in IAANS fluorescence as Ca²⁺ was removed by EGTA from control , and the HCM mutants, TnT R92Q and TnI S166F . Panel C shows the time courses of the increase in IAANS fluorescence as Ca²⁺ was removed by EGTA from control , and the RCM mutants, TnI D190H and TnI R192H . Panel D shows the time courses of the increase in IAANS fluorescence as Ca²⁺ was removed by EGTA from control and ischemic related truncated TnI (1-192) . All complexes consist of the full length Tn subunits of , TnI and TnT, except for ischemic related truncated TnI (1-192). The disease related modification is either in TnI or TnT, in either case, the other protein (TnT or TnI) was wild type. Data traces (an average of 3 to 5 individual traces collected at least 10 times) were fit with a single exponential equation to calculate the kinetic rates. The data traces have been staggered and normalized for clarity.

Inline graphic — Panel A shows the time courses of the increase in IAANS fluorescence as Ca²⁺ was removed by EGTA from control , and the DCM mutants, TnI K36Q , TnT R131W , TnT R141W , TnT R205L and TnT ΔK210 . Panel B shows the time courses of the increase in IAANS fluorescence as Ca²⁺ was removed by EGTA from control , and the HCM mutants, TnT R92Q and TnI S166F . Panel C shows the time courses of the increase in IAANS fluorescence as Ca²⁺ was removed by EGTA from control , and the RCM mutants, TnI D190H and TnI R192H . Panel D shows the time courses of the increase in IAANS fluorescence as Ca²⁺ was removed by EGTA from control and ischemic related truncated TnI (1-192) . All complexes consist of the full length Tn subunits of , TnI and TnT, except for ischemic related truncated TnI (1-192). The disease related modification is either in TnI or TnT, in either case, the other protein (TnT or TnI) was wild type. Data traces (an average of 3 to 5 individual traces collected at least 10 times) were fit with a single exponential equation to calculate the kinetic rates. The data traces have been staggered and normalized for clarity.