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. 2012 Jun 4;7(6):e36996. doi: 10.1371/journal.pone.0036996

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Panel (A) Coomassie blue stained SDS-PAGE gel analysis of maleimide-EPA (lanes 1 and 5); monomeric MSP1₄₂-FUP (lanes 2 and 6), un-purified conjugation mixture of MSP1₄₂-FUP-EPA_APA (lane 3), bulk purified MSP1₄₂-FUP-EPA_APA (lane 4); un-purified conjugation mixture of MSP1₄₂-FUP-EPA_PEO (lane 7) and bulk purified MSP1₄₂-FUP-EPA_PEO (lane 8). The asterisks indicate the conjugates with 3∶1 and 4∶1 ratio. Panel (B) SEC-HPLC-MALS analysis. Solid and dashed lines represent absorbance at 280 nm and molecular mass for MSP1₄₂-FUP-EPA_PEO and MSP1₄₂-FUP-EPA_APA, respectively. The capital letters A, B and C indicate the profile peaks of each conjugate.