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. 2012 Jun 4;7(6):e38393. doi: 10.1371/journal.pone.0038393

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This is an open-access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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IHC was performed on Ank and Enpp1 ^+/+ (Control) and ^−/− tissues at 26 dpn. OPN defines the acellular cementum layer in (A) wild-type cervical cementum, and is intensely localized to the thick AEFC in (B, C) both ^−/− models. DMP1 did not label acellular cementum in (G) ^+/+ controls, but was increased dramatically in the thickened cervical cementum of (H, I) both ^−/− models. BSP was present in (M) control AEFC, as well as in (N, O) Ank and Enpp1 ^−/− AEFC in diluted concentrations. Localization of OPN, DMP1, and BSP was not different in cellular cementum of null models vs. controls (D–F, J–L, and P–R). Abbreviations: d = dentin; c = acellular cementum; p = periodontal ligament; b = bone. Scale bar = 100 µm.