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. 2012 Jun 4;7(6):e37845. doi: 10.1371/journal.pone.0037845

Figure 4. Identification of LmrCD-activating DARPins.

Figure 4

(A) Overexpression of DARPin_Act1 (•), DARPin_Act2 (○), DARPin_Act3 (▾) in wildtype L. lactis increases the resistance towards daunomycin compared to cells expressing control DARPin E3_5* (not interacting with LmrCD) (Δ). (B) No differences were observed when experiments in (A) were performed with cells lacking the chromosomal copy of lmrCD. (C) BCECF-AM transport measurements in pre-energized wildtype L. lactis cells demonstrate activation of LmrCD-mediated extrusion upon expression of DARPin_Act2 (trace 1) but not of control DARPin E3_5* (trace 2). No activation of LmrCD activity was observed upon expression of DARPin_Act2 (trace 3) or control DARPin E3_5* (trace 4) in L. lactis ΔlmrCD cells. Shown are representative data from at least three independent measurements (n≥3).