| pOI277 |
pACYC184 containing lacI
q and 6his-etk under control of the tac promoter |
[11]
|
| pMS3239 |
pOI277 expressing the K545M inactive mutant |
This study |
| pSJ8 |
pET21a with MBP-8His-(TeV protease cleavage site)- upstream of cloning site |
Zhaohui Xu |
| pKS2084 |
pSJ8 expressing MBP-8His-TeV-Etk(446–726), amplified from pOI277 with primers 207 and 45 |
This study |
| pET28b |
Expression vector with T7 promoter |
Novagen |
| pMS0125 |
pET28b expressing 6His-TeV-Etp |
This study |
| pAJ0046 |
pMS0125, expressing 6His-EtpD119A |
This study |
| pAJ0049 |
pMS0125, expressing 6His-EtpY121F |
This study |
| pAJ2090 |
pMS0125, expressing 6His-EtpD119E |
This study |
| pSA10 |
Expression vector with tac promoter |
[24]
|
| pCNY506 (2723) |
pSA10 carrying etp, with 80 bp upstream region and 6His at C-terminus. |
This study |
| pCNY507 (2790) |
pCNY506, expressing EtpD119A |
This study |
| pAJ3242 |
pCNY506, expressing EtpD119E |
This study |
| pCNY508 (2792) |
pCNY506, expressing EtpY121F |
This study |
| pCNY512 (3139) |
pCNY506, expressing EtpY121E |
This study |
| pCNY513 (3152) |
pCNY506, expressing EtpY121A |
This study |
| pCNY510 (2967) |
pCNY506, expressing EtpD119A, Y121F |
This study |
| pAP406 |
pQE31 containing lacI
q and expressing 6His-Etp |
[8]
|
| pKD4 |
Template for amplification of Kan cassette |
[22]
|
| pKD46 |
Temperature-sensitive plasmid encoding lambda Red genes |
[22]
|
