Table 4. Oligonucleotide primers used in this study.
| No. | Primer Sequence (5′ to 3′)* | Use |
| 424 | GCGAATTCCCGCTGGCTCGTTGGAACCG | Forward primer for creating pCNY506 |
| 425 | GCGTCGACTTAGTGATGGTGATGGTGATGCCGGCTGAGGCGCTTCGC | Reverse primer for creating pCNY506 |
| 225 | CTATCCGCATATGGCCCAACTAAAATTTAAC | Forward primer for creating pMS0125 |
| 226 | CGACTCGAGTTACCGGCTGAGGCGCTTCG | Reverse primer for creating pMS0125 |
| 207 | CGTGGGATCCCGTGCGATGTTGCGTCGTG | Forward primer for creating pKS2084 |
| 45 | GATCTCGAGTCACTCTTTCTCGGAGTAACTATAAC | Reverse primer for creating pKS2084 |
| 263 | ACCGTCAATGGTATGGTC | Forward primer for creating etk::kan in AP1417 |
| 264 | CGTTTTACCACTGTCTGG | Reverse primer for creating etk::kan in AP1417 |
| 475 | CGGGAAACGTTTGCAGCGGTGTACACATTACTTGAACGGTCTGCCCGCCAcaagagggtcattatatttcg | Forward primer for creating Δwzc::tet |
| 476 | AGGTAGGTCGGATAAGATGCGTCAGCATCGCATCCGACAGCGGATGTCGactcgacatcttggttaccg | Reverse primer for creating Δwzc::tet |
| 480 | GAAAGAGATCCCGGCTCCCTATCGTAAAAG | Forward primer for EtpD119A substitution in pCNY507 |
| 481 | CTTTTACGATAGGGAGCCGGGATCTCTTTC | Reverse primer for EtpD119A substitution in pCNY507 |
| 298 | GATCCCGGATCCCTTTCGTAAAAGTCAGG | Forward primer for EtpY121F substitution in pCNY508 |
| 299 | CCTGACTTTTACGAAAGGGATCCGGGATC | Reverse primer for EtpY121F substitution in pCNY508 |
| 460 | GAAAGAGATCCCGGCTCCCTTTCG TAAAAG | Forward primer for EtpD119A, Y121F substitutions in pCNY510 |
| 461 | CTTTTACGAAAGGGAGCCGGGATC TCTTTC | Reverse primer for EtpD119A, Y121F substitutions in pCNY510 |
| 437 | GAGATCCCGGATCCCGAGCGTAAAAGTCAGGACG | Forward primer for EtpY121E substitution in pCNY512 |
| 438 | CGTCCTGACTTTTACGCTCGGGATCCGGGATCTC | Reverse primer for EtpY121E substitution in pCNY512 |
| 493 | GAGATCCCGGATCCCGCGCGTAAAAGTCAGGACG | Forward primer for EtpY121A substitution in pCNY513 |
| 494 | CGTCCTGACTTTTACGCGCGGGATCCGGGATCTC | Reverse primer for EtpY121A substitution in pCNY513 |
| 58 | CAGAAAGAGATCCCGGCTCCCTATCGTAAAAGTCAGG | Forward primer for EtpD119A substitution in pAJ0046 |
| 59 | CCTGACTTTTACGATAGGGAGCCGGGATCTCTTTCTG | Reverse primer for EtpD119A substitution in pAJ0046 |
| 54 | GAGATCCCGGATCCCTTTCGTAAAAGTCAGGACG | Forward primer for EtpY121F substitution in pAJ0049 |
| 55 | CGTCCTGACTTTTACGAAAGGGATCCGGGATCTC | Reverse primer for EtpY121F substitution in pAJ0049 |
| 49 | CAGAAAGAGATCCCGGAGCCCTATCGTAAAAGTCAGG | Forward primer for EtpD119E substitution in pAJ2090, pAJ3242 |
| 48 | CCTGACTTTTACGATAGGGCTCCGGGATCTCTTTCTG | Reverse primer for EtpD119E substitution in pAJ2090, pAJ3242 |
*
Underlined sequences represent restriction enzyme sites, and lower case sequences are regions complementary to the template plasmid containing the antibiotic cassette.