Figure 4.
miR-320 does not decrease Sema3a in N2A cells. Immunoblots of N2A cells treated with either (a,b) pri-miR-320 (n = 6 cultures/group, p = 0.62) or (c,d) miR-320 mimic (n = 4 cultures/group, p = 0.12) and probed with an antibody against Sema3a. (e-f) Representative image of a DotBlot (e) and quantification (f) of the levels of Sema3a in medium of cells transfected with control or miR-320 mimic (n = 3 cultures/group, p = 0.64).