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. Author manuscript; available in PMC: 2013 Aug 1.
Published in final edited form as: Neurobiol Dis. 2012 Apr 11;47(2):194–200. doi: 10.1016/j.nbd.2012.03.040

Figure 1. ABC drug efflux transporter expression levels in SOD1-G93A mice.

Figure 1

Isolated messenger RNA from the lumbar spinal cord of SOD1-G93A mice shows an increase in ABCG2 (Bcrp) and ABCB1B (P-gp) with no change in other ABC transporters, the endothelial cell marker (Glut1), and a member of the organic anion transporters (OATP2) between presymptomatic (50 days old) and symptomatic mice (130 days old). (A) PCR drug transporter array identifying increases in the mRNA coding for two ABC drug efflux transporters with localization to the blood-spinal cord barrier (P-gp and BCRP) throughout disease progression in the SOD1-G93A mouse model. (B) qRT-PCR validation of ABCB1B, ABCG2, and ABCC2 which confirm results found in the array. Analysis was run as three independent samples in triplicate per time point (n=3) with values represented using the ΔΔCt method as fold-change using an internal control. Statistical analysis was done using Student’s T-test and compared ΔCT values along with the corresponding standard errors. *P < 0.05, data are shown as mean ± SEM.