. Author manuscript; available in PMC: 2013 Jun 18.

Published in final edited form as: J Proteomics. 2012 Apr 6;75(11):3342–3350. doi: 10.1016/j.jprot.2012.03.043

Table 2.

Summary of the effect of insulin on PPP1R12A phosphorylation

Start	Stop	Peptide Sequence	Phosphor-Site	Fold Change ^a	Log2 fold change
17	30	WIGpSETDLEPPVVK	Ser20	1.88±0.20	0.91
296	315	DKKpSPLIESTANMDNNQSQK	Ser299	0.99±0.16	−0.01
296	315	DKKSPLIEpSTANMDNNQSQK	Ser304	1.00±0.17	0.00
421	428	IpSPKEEER	Ser422	0.85±0.17	−0.23
443	456	pTGSYGALAEITASK	Thr443	0.97±0.16	−0.04
443	456	TGpSYGALAEITASK	Ser445	1.24±0.06	0.31
476	483	LpSSSLDNK	Ser477	10.92±2.97^*	3.45
476	483	LSpSSLDNK	Ser478	4.84±1.39^*	2.28
505	514	LApSTSDIEEK	Ser507	6.53±1.37^*	2.71
505	514	LASTpSDIEEK	Ser509	0.48±0.08^*	−1.06
504	514	RLApSTpSDIEEK	Ser507, Ser509	3.49±0.61^*	1.80
668	682	pSYLTPVRDEESESQR	Ser668	2.25±0.38^*	1.17
668	682	SYLpTPVRDEESESQR	Thr671	0.66±0.06	−0.60
668	682	SYLTPVRDEEpSESQR	Ser678	0.60±0.11	−0.74
695	709	pSTQGVTLTDLQEAEK	Ser695	2.38±0.18^*	1.25
908	917	SGpSYSYLEER	Ser910	1.19±0.20	0.25
993	1002	RIpSEMEEELK	Ser995	1.00±0.11	0.00

Fold change: obtained by dividing the normalized peak area for a phosphopeptide in each experiment by the average of the normalized peak area under the control condition, n=6.

Indicate insulin stimulated or suppressed phosphorylation at these sites by more than 2-fold.