Table 3.
Potential kinases for PPP1R12A phosphorylation sites
| Start | Stop | Peptide Sequence | Phosphor-Site | Predicted Kinases |
|---|---|---|---|---|
| 17 | 30 | WIGpSETDLEPPVVK | Ser20 | CSNK2A2, CK2A1 |
| 296 | 315 | DKKpSPLIESTANMDNNQSQK | Ser299 | CDK2, CDK3 |
| 296 | 315 | DKKSPLIEpSTANMDNNQSQK | Ser304 | NEK2, DMPK |
| 421 | 428 | IpSPKEEER | Ser422 | CDK2, CDK3 |
| 443 | 456 | pTGSYGALAEITASK | Thr443 | DMPK, AURKB, NEK2 |
| 443 | 456 | TGpSYGALAEITASK | Ser445 | PRKAA1, PRKAA2, NUAK1 |
| 476 | 483 | LpSSSLDNK | Ser477* | PAK4, PAK6, PAK7 |
| 476 | 483 | LSpSSLDNK | Ser478* | CSNK2A2, CK2A1 |
| 505 | 514 | LApSTSDIEEK | Ser507* | CSNK2A2, CK2A1 |
| 505 | 514 | LASTpSDIEEK | Ser509* | CSNK2A2, CK2A1 |
| 668 | 682 | pSYLTPVRDEESESQR | Ser668* | RPS6KB1, RPS6KB2, PAK4, PAK6, PAK7, DMPK, Pim1/2, CLK1, CLK2 |
| 668 | 682 | SYLpTPVRDEESESQR | Thr671 | CDK2, CDK3 |
| 668 | 682 | SYLTPVRDEEpSESQR | Ser678 | CSNK2A2, CK2A1, TGFBR2, ACVR2A, ACVR2B |
| 695 | 709 | pSTQGVTLTDLQEAEK | Ser695* | PKA, PKG, PIM2 |
| 908 | 917 | SGpSYSYLEER | Ser910 | AKT1, AKT2, AKT3, PAK4, PAK6, PAK7, NUAK1 |
| 993 | 1002 | RIpSEMEEELK | Ser995 | CSNK2A2, CK2A1 |
Insulin stimulated or suppressed phosphorylation at these sites by more than 2-fold.
Using NetworKIN 2.0 Beta (http://networkin.info/version_2_0/newPrediction.php) and additional literature search.
Kinase abbreviations: Activin receptor type IIA/B precursor (ACVR2A/B), RAC serine/threonine-protein kinase (AKT1/2/3), Serine/threonine-protein kinase aurora-B (AURKB), Cell division protein kinase 2/3 (CDK2/3), casein kinase 2, alpha 1 polypeptide (CK2A1), casein kinase 2, alpha prime polypeptide (CSNK2A2 or CK2A2), Dual specificity protein kinase CLK1/2 (CLK1/2), Myotonin-protein kinase (DMPK), Serine/threonine-protein kinase Nek2 (NEK2), AMPK-related protein kinase 5 (NUAK1), Serine/threonine-protein kinase PAK 4/6/7 (PAK4/6/7, also known as p21-activated kinase 4/6/7), cAMP-dependent protein kinase (PKA), cGMP-dependent protein kinase (PKG), Proto-oncogene serine/threonine-protein kinase Pim-1/2 (PIM1/2), 5′-AMP-activated protein kinase, catalytic alpha-1/2 chain (PRKAA1/2 or AMPK alpha-1/2), Ribosomal protein S6 kinase 1/2 (RPS6KB1/2), TGF-beta receptor type II precursor (TGFBR2).