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. Author manuscript; available in PMC: 2013 Aug 1.
Published in final edited form as: J Microbiol Methods. 2012 May 2;90(2):80–82. doi: 10.1016/j.mimet.2012.04.019

Fig. 1.

Fig. 1

Reporter phage-mediated bioluminescent detection of Y. pestis. A. Signal response time and signal strength. The ability of the first and second generation (1st gen., and 2nd gen.) reporter phages to transduce a bioluminescent signal response to Y. pestis was compared. Y. pestis was grown in Luria Bertani broth at 28°C to an A600 of approximately 0.2. At time 0, cells (~1 × 108 CFU/mL) were mixed with the reporter phage (multiplicity of infection [MOI] of 0.5) and incubated at 28°C. Bioluminescence (RLU) was measured over time following the addition of the substrate n-decanal. Numbers are the mean (n=3) ± SD. B. Dose-dependent detection. 10-fold serial dilutions of a Y. pestis culture were mixed with the second generation reporter phage and incubated at 28°C. Bioluminescence (RLU) was measured over time following the addition of n-decanal. Numbers are the mean (n=3) ± SD.