Skip to main content
. Author manuscript; available in PMC: 2013 Jun 1.
Published in final edited form as: J Pain. 2012 May 19;13(6):598–607. doi: 10.1016/j.jpain.2012.03.011

Figure 1.

Figure 1

Clonidine induced conditioned place preference (CPP) in CFA-, but not saline-, pretreated mice.

(A-B). Complete Freund’s adjuvant (CFA) produced mechanical (A) and thermal hypersensitivity (B), which were transiently attenuated by clonidine (1 μg in 5 μL saline, i.t.). The sensory baseline (D0) was obtained before the injection of CFA on day 0. Post-CFA data were obtained 24 h later (D1). After the administration of clonidine, mechanical and thermal sensitivity were monitored for 120 min. * p<0.05, ** p<0.01, *** p < 0.001, compared between CFA-clonidine and CFA-saline groups;. # p<0.05, ## p<0.01, ### p < 0.001, compared with the saline-saline group, one-way ANOVA followed by Tukey post hoc test; n = 8 for each group. (C). Clonidine induced CPP in CFA-pretreated mice. After 3 d pre-conditioning, mice were tested to ensure the absence of chamber bias (“pre”). CFA-pretreated mice (“CFA”) spent significantly longer time in clonidine-paired chamber, whereas saline-pretreated mice (“saline”) showed no chamber preference. *** p < 0.001, compared between the saline paired and clonidine paired group, two-way ANOVA followed by Bonferroni post hoc test; n = 8 for each group.

(D) Difference scores (test time-preconditioning time spent in the clonidine chamber) confirmed that CFA-, but not saline-, pretreated mice showed CPP to clonidine. ** p < 0.01, paired t test; n = 8 for each group.