Figure 3.
The specific detection of two tmRNA species on a continuous single chip assay. Microrings were functionalized with either Streptococcus pneumonia (black) or Streptococcus agalactiae (red) tmRNA-specific DNA oligonucleotide capture probes. tmRNA from four different bacterial species (Klebsiella pneumoniae, Enterococcus faecium, S.agalactiae, and S.pneumoniae) were sequentially introduced into the flow chamber, and a specific response observed for each tmRNA. Washing steps with neat buffer solution were carried out between hybridization steps.