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. Author manuscript; available in PMC: 2013 Jun 1.
Published in final edited form as: Cancer Res. 2012 Apr 3;72(11):2768–2779. doi: 10.1158/0008-5472.CAN-11-3567

Fig. 3. Paracrine signaling of cancer-secreted cytokines induces CCL2 production in fibroblasts via STAT3 activation.

Fig. 3

(A) A luciferase reporter containing a 2.8-kb CCL2 promoter was transfected into CAF265922 cells. Luciferase activity was analyzed at 4 h post CM exposure in the presence of DMSO or Stattic (a STAT3 inhibitor; 5 μM). Each bar represents the mean ± S.D. of 3 independently transfected wells. * p<0.01 compared to the control (the first column). (B) Total RNA isolated from CAF265922 that had been treated with CM from indicated BC cells for 4 or 24 h was analyzed for CCL2 mRNA level by RT-qPCR. Data were normalized to 18S in each sample. Each bar represents the mean ± S.D. of 3 wells. * p<0.01 compared to the control (the first column). (C) CAF265922 cells were treated with CM from indicated cells and analyzed by Western blot. (D) Summary of the cytokine array data identifying cytokines constitutively secreted by BT474 and MDA361 BC cells. Cytokines that are known to activate STAT3 are in bold.