Fig. 2.

High-throughput screen for small-molecule inhibitors of cardiac ADPR cyclase. 17,567 compounds were tested at 10 μM for inhibition of pig cardiac ADPR cyclase. Autofluorescent compounds with fluorescence at t = 0 are shown in light grey and were removed before further analysis (327 compounds). Horizontal black lines denote the mean percent inhibition value over all compounds ± standard deviation that was determined as −3.2 ± 13.8. Non-fluorescent compounds with a percent inhibition above mean + 3 SD were considered as screening hits (93 compounds)