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. 2012 Apr 1;53(6):1154–1170. doi: 10.1093/pcp/pcs043

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© The Author(s) 2012. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 2 — Equipment for hyperspectral reflectance imaging and data analysis. The programs shown in B–D are provided as Supplementary File S1 of this article (see Supplementary Text S1 for legends and methods of operation). (A) Hyperspectral image acquisition system. The system is basically composed of a hyperspectral camera directed downward to the sample stage and epi-illumination halogen lights. The inset shows the VIS/NIR hyperspectral camera HSC1700 (early 2007 model). (B) Screenshot of HSD Analyzer software. The software facilitates calibration and extraction of numerical reflectance data from the areas of interest in hyperspectral images. (C) Screenshot of PPM software. The software allows visualization of individual concentrations and compositional parameters of major pigments in Arabidopsis leaves. (D) Screenshot of HSD Visualizer software. The software displays the deviation of spectral reflectance relative to the reference spectra (usually from the wild type) as a pseudocolor image.