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Analysis of bias in m5C candidate site location within mRNA. (A) Histogram showing relative enrichment of m5C sites in 3′ UTR and 5′ UTR compared to CDS (Error bars = 95% confidence interval; ‘Poisson’ distribution). (B) RNA-binding protein target density versus distance from m5C site in protein coding transcripts (CDS and UTR both). Confidence bands are shown in grey (SEM).
