Figure 3.

Demonstration of a novel long-range interaction within the DLS. (A) DLS sequence alignment of nine HIV-1 subtypes and SIVcpz. PBS and DIS are presented as shaded. The evolutionarily-conserved complementary sequences are in blue boxes (GCGUC) and green boxes (GACGC). (B) The sequence of base substitution mutants. The bases for mutation are shown in bold. The G to U substitutions at the 3′-end are an inevitable change for silent mutation for S4m3, although the G is not included in the DLS in this report. Thus, we introduced a 4-base substitution in the compensatory mutant (S4m3PK). (C) Dimerization ability of the mutants. Results are the average of at least three independent experiments with SEM. (D) Replication of DLS mutants. Growth curves of two sets of 1-base substitution and compensatory mutants are shown. Data are representative of three independent experiments.