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. 2012 Feb 22;40(11):4861–4878. doi: 10.1093/nar/gks162

Figure 7.

Figure 7.

Sperm chromatin remodeling activity of the NPM1–NPM3 complex. (A) Sperm chromatin decondensation activity of the NPM1–NPM3 complex. Sperm nuclei in the absence or presence of His–NPM1, NPM1–His–NPM3 complex (NPM1/3), His–NPM3 prepared as in Figure 6C were incubated for 30 or 60 min. Sperm DNA was fixed and stained with DAPI, and observed by fluorescent microscopy. Three typical sperms (from 60 min incubation samples) are shown for each sample. Sperm nuclear size (n > 30) was estimated by Image J and averaged. Results are means ± SD. (B) Nucleosome assembly activity of the NPM1–NPM3 complex. Increasing amounts of His–NPM1, NPM3, and the NPM1–His–NPM3 complex (NPM1/3) (200, 600 and 2000 ng, for lanes 2–4, 5–7 and 8–10) were mixed with core histones (100 ng). Topo I-treated plasmid DNA (100 ng) was added and further incubated. DNA was purified, separated on 1% agarose gel and visualized with GelRed staining. Positions of relaxed (R) and supercoilied (S) DNA are indicated at the right side of the panel.