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. 2012 Feb 22;40(11):5052–5064. doi: 10.1093/nar/gks164

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© The Author(s) 2012. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — GMS assays with rCRR22 and target RNAs. (A) RNA sequences used as probes are shown. Editing sites of ndhB-7, ndhD-5 and rpoB-3 are indicated in bold and are marked with arrowheads. (B) GMS assays were performed with the indicated concentrations of rCRR22 and labeled RNAs (ndhB7L, ndhB7S-S3, ndhD5L, ndhD5S-S3, rpoB3L and rpoB3S-S3), as described in the ‘Materials and Methods’ section. (C) Equilibrium K_d of rCRR22 for the ndhB7S-S3 (left), ndhD5S-S3 (middle) and rpoB3S-S3 (right) probes. rCRR22 concentrations and fractions of RNA bound in each lane are plotted. The K_d calculation assumes a 1:1 interaction between the RNA and the protein. The K_d values and each data point are means ± SD of three experiments performed with the same rCRR22 preparation. All of the GMS assays were performed with the same preparation of rCRR22 and within 2 weeks after purification.