Skip to main content
. 2012 Feb 28;40(11):4988–4997. doi: 10.1093/nar/gks184

Figure 5.

Figure 5.

DNA bending. Looping dynamics were measured for three DF constructs with inter-site spacings of 181, 185 and 190 bp and compared to their IF counterparts: both loop capture (A) and loop release (B) rates are plotted as a function of the number of base pairs between the recognition sites. In both panels, rates for the DF constructs are shown in dark grey and their IF counterparts in light grey: error bars indicate SEMs. In (A), the histograms for the loop capture rates demonstrate that the IF and DF substrates are out of phase with each other. Loop capture rates for IF181 and IF190 are both slow, while that for IF185 is fast while the DF constructs display the reverse behaviour, fast capture for DF181 and DF190 and slow for DF185. In contrast, the loop release rates in (B) show the same trends for the IF and the DF substrates. In both the IF and the DF constructs, the 185-bp spacing gave a faster loop release rate than either the 181- or the 190-bp site separations.