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. 2012 Feb 8;40(11):e85. doi: 10.1093/nar/gks213

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© The Author(s) 2012. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — Transient and stable transfection of Ba/F3 cells using SB transposon system. (A) Comparison of transient transfection efficiency by nucleofection delivery of plasmid and minicircle bearing T3/eGIF transposon. Ba/F3 cells were nucleofected with 5 µg of either plasmid pT3/eGIF or minicircle mcT3/eGIF. Flow cytometry was performed 24 h after nucleofection. (B) Integration of T3/eGIF transposon. Ba/F3 cells were nucleofected with varying DNA amounts of transposon minicircle mcT3/eGIF and transposase plasmid pSB100x. Flow cytometry was performed 24 h (gray bars) and 7 days (striped bars) after nucleofection to assess transient and stable transfection efficiency, respectively. Bars represent SD of three independent transfections. Averages of mean fluorescence intensity of GFP⁺/PI⁻ populations are indicated above the graphs in RFU.