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. 2012 Mar 13;40(11):e87. doi: 10.1093/nar/gks230

Figure 2.

Figure 2.

Example template sequence #1 (11) consisting of (A) the trigger reverse complement X′, which is replicated at positions 1–10 and 21–30, (B) the nicking enzyme post-cut site, (C) the reverse complement of the nicking enzyme recognition site, (D) an additional T at the end of the trigger-binding site.