Figure 2.

Hypoxia results in Fe³⁺ deposition and hydroxyl radical generation. (A) Representative images of Perl's staining in the CA1 and CA3 regions of hippocampus. Scale bars shown in μm. (B) Graph showing Fe²⁺/Fe³⁺ ratio in hippocampal samples as estimated biochemically. The concentration of Fe²⁺/Fe³⁺ was determined from a standard curve. (C) Graph depicting concentration of hydroxyl radical as determined by high-performance liquid chromatography. Concentration of reactive oxygen species (ROS) was determined indirectly by calculating the area of plot for the ROS metabolite 2,3-DHBA. ^*Denotes P<0.01 when compared with normoxia and # denotes P<0.01 when compared with corresponding NAC-untreated group. H, hypoxia; NAC, N-acetyl cysteine; DFO, desferrioxamine; DHBA, dihydrobenzoic acid.