Figure 5.

Neuroglobin (Ngb) upregulation decreases chromatin condensation and apoptosis in N2a cells exposed to hypoxia. (A) Representative images of normoxic, normoxic+Ngb-silenced, normoxic+Ngb overexpressed, hypoxic, hypoxic+Ngb-silenced, and hypoxic+Ngb overexpressed N2a cells. Scale bar denotes 10 μm. (B) Corresponding graph denotes percentage of cells showing chromatin condensation that was determined by counting mean±s.e.m. of number of cells with chromatin condensation and considering normoxic values to be 100%. ^*Denotes P<0.01 when compared with normoxic cells. (C) Representative flow cytometric images of normoxic, normoxic+Ngb overexpressed, normoxic+Ngb-silenced, hypoxic, hypoxic+Ngb overexpressed, and hypoxic+Ngb-silenced N2a cells. Apoptosis was determined based on Annexin V and necrosis was determined by propidium iodide staining. NAC, N-acetyl cysteine; Si, silenced; OE, overexpressed.