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. 2012 Mar 23;13(6):547–553. doi: 10.1038/embor.2012.40

Figure 1.

Figure 1

Neurl4 is a novel NHR domain-containing protein that interacts with CP110. (A) Western blot detection of endogenous Neurl4. U2OS cells were transfected with a nonspecific control (NS) or two different Neurl4-specific siRNA as indicated. (B) 293T cells transfected with empty vector (EV) or Flag–Neurl4 were immunoprecipitated with rabbit IgG (control), anti-Neurl4 or anti-CP110 antibody and blotted with indicated antibodies. (C) Western blotting of endogenous CP110, Cep97, Cep76, Cep290 and Neurl4 after immunoprecipitation (IP) with indicated antibodies from 293T cell extracts. IgG, immunoglobulin G; siRNA, small interfering RNA.