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. 2012 Mar 23;13(6):547–553. doi: 10.1038/embor.2012.40

Figure 2.

Figure 2

Neuralized homologue 4 (Neurl4) preferentially localizes to the daughter centriole. (A) U2OS cells in diverse stages of the cell cycle were visualized by immunofluorescence with Neurl4 (red) and centrin (green) antibodies. (B–D) U2OS cells were visualized with Cep170 (green) and Neurl4 (red) antibodies (B) or Sas6 (green) and Neurl4 (red) antibodies (C). Quiescent retinal pigment epithelial 1 cells were stained with Neurl4 (red) and polyglutamylated tubulin (GT335, green) antibodies (D). Cell cycle stages for each cell are indicated. (E) Plk4 expression in U2OS cells produces a ‘rosette’ pattern with daughter centrioles, stained with Neurl4 antibodies, surrounding an unstained mother centriole in the centre. (F–H) Triple staining of Neurl4 (red)/centrobin (green)/centrin 2 (blue; F), Neurl4 (red)/cenexin (green)/centrin 2 (blue; G) and Neurl4 (red)/CP110 (green)/centrin 2 (blue; H). Scale bar, 2.5 μm.