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. 2012 May 8;13(6):561–568. doi: 10.1038/embor.2012.58

Figure 4.

Figure 4

NBS1 S432A impairs replication restart. (A) Cells were treated for 24 h with the indicated HU dose. Data represent the average of three independent experiments (±s.d.). (B) Mitotic entry after HU release in complemented NBS and (C) ATLD2 cells. Cells were treated with 2 mM HU for 24 h. Data represent the average of three independent experiments (±s.d.). (D) Replication restart after aphidicolin treatment in complemented NBS cells. (E) Quantification of IF data in complemented NBS and (F) ATLD2 cells. At least 100 cells were counted per condition. Error bars represent standard deviations. ATLD2, Ataxia telangiectasia-like disease; CldU, 5-chloro-2-deoxyuridine; HR, homologous recombination; HU, hydroxyurea; IdU, 5-iodo-2-deoxyuridine; IF, immunofluorescence; IR, ionizing radiation; NBS, Nijmegen breakage syndrome; RPA, replication protein A; UV, ultraviolet light; WT, wild-type.